Journal: Journal of Cellular and Molecular Medicine

Article Title: Bmi1 regulate tooth and mandible development by inhibiting p16 signal pathway

doi: 10.1111/jcmm.16468

Figure Lengend Snippet: p16 knockout promoted dentin maturation and formation in Bmi1‐deficient mice. Paraffin‐embedded sections through the first molars and the incisors from 4‐wk‐old wild‐type (WT), p16 −/− , Bmi1 −/− and Bmi1 −/− p16 −/− mice stained with (A) HE, immunohistochemically for (B) biglycan and (C) dentin sialoprotein (DSP) and photographed. Scale bars represent 50 μm. (A) Representative HE staining of micrographs of the root walls of the first molars. (D) Quantitative thickness of predentin in the root walls of the first molars. (B) Representative micrographs of the root wall of the first molars stained immunohistochemically for biglycan, and (C) the root wall of the first molars stained immunohistochemically for DSP. (E) Quantitative biglycan immunopositive areas in the first molars. (F) Quantitative DSP immunopositive areas in the first molars. Each value is the mean ± SEM of determinations in five animals of each group. ** P < .01; *** P < .001 relative to the wild‐type mice. # P < .05 compared with Bmi1 −/− mice

Article Snippet: Immunohistochemical staining was carried out for biglycan and dentin sialoprotein (DSP), using the avidin‐biotin‐peroxidase complex technique with affinity‐purified rabbit anti‐mouse biglycan antibody (1:400, ab58562, Abcam, Cambridge, UK) and dentin sialoprotein (1:200, sc‐33587, Santa Cruz, CA, USA) following previously described methods.

Techniques: Knock-Out, Staining

Journal: American Journal of Translational Research

Article Title: Dspp mutations disrupt mineralization homeostasis during odontoblast differentiation

doi:

Figure Lengend Snippet: Oligonucleotide primers for real-time RCR with expected fragment size

Article Snippet: After blocking with TBST containing 5% nonfat dry milk the blots were detected by specific commercial antibodies diluted in TBST overnight at 4°C: DSP (Santa Cruz Biotechnology, sc-33587, USA), Bmp2 (Abcam, ab82511, UK), Col1 (Abcam, ab88147, UK), Runx2 (Abcam, ab76956, UK), Ocn (Santa Cruz Biotechnology, sc-376835, USA), Mgp (Santa Cruz Biotechnology, sc-66965, USA), Htra1 (Abcam, ab38611, UK), and β-actin (Epitomics, USA).

Techniques: